The popularity of vaping electronic cigarettes (e-cigarettes) containing nicotine has grown rapidly over the last decade in the young adult population, yet, the long-term health consequences remain unknown. Existing research has focused on examining the impacts of acute or sub-acute vaping in humans or chronic exposure using animal models. The purpose of our research is to examine the potential physiological changes in vascular health that are caused due to self-elicited chronic exposure to vaping in a young, apparently healthy population. The prosed project will expand on preliminary data, which showed no significant differences in the degree of arterial stiffness between never-smokers and vapers who were physically active. Based on these preliminary data, it is unclear if an increased level of cardiovascular fitness acted as a protective mechanism against arterial stiffening or if the exposure to e-cig aerosol was not long enough to elicit significant changes (average months vaping = 19.9+11.7 months) warranting the need for further research. Building on the preliminary research, we will be examining additional metrics related to vascular health including pulse wave velocity, subendocardial viability ratio, brachial and central blood pressure, augmentation index, and augmentation pressure between never-smokers and vapers. Vapers will include individuals who have exclusively vaped for at least 36 months and do not have a history of tobacco cigarette smoking. We will also analyze if differences in vascular biometrics are independent of aerobic fitness status in this sample population. The proposed research will provide the broader scientific and healthcare community insight into the effects of vaping e-cigarette aerosol. Lastly, participating young adults will gain valuable information on their vascular health through biometric monitoring using the SphygmoCor® technology, enabling them to make informed decisions that may impact their lifetime.